buffer 1 Search Results


acetate buffer  (Thermo Fisher)
95
Thermo Fisher acetate buffer
Acetate Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acetate buffer/product/Thermo Fisher
Average 95 stars, based on 1 article reviews
acetate buffer - by Bioz Stars, 2026-06
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wash buffer  (R&D Systems)
95
R&D Systems wash buffer
Wash Buffer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
wash buffer - by Bioz Stars, 2026-06
95/100 stars
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anti ghr1  (R&D Systems)
93
R&D Systems anti ghr1
Anti Ghr1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ghr1/product/R&D Systems
Average 93 stars, based on 1 article reviews
anti ghr1 - by Bioz Stars, 2026-06
93/100 stars
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d rehydration  (Bio-Rad)
93
Bio-Rad d rehydration
D Rehydration, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d rehydration/product/Bio-Rad
Average 93 stars, based on 1 article reviews
d rehydration - by Bioz Stars, 2026-06
93/100 stars
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cetsa cell lysis buffer 2  (Revvity)
91
Revvity cetsa cell lysis buffer 2
(A) Schematic of AlphaLISA-based <t>CETSA.</t> GLS engagement by CB-839 protects it from thermal denaturation, which allows it to be recognized by GLS antibodies in 384-well plates. Secondary antibodies conjugated to donor and acceptor beads recognize the Fc domain of GLS antibodies, and all together form a complex. When donor beads are excited at 680nm, they emit singlet oxygen; the close complex formed by donor bead-antibody-GLS-antibody-acceptor bead allows the singlet oxygen to excite the acceptor bead, and an Alpha signal is emitted at 615nm. In contrast, if no CB-839 is present to engage with GLS, GLS is thermally denatured and precipitates, and cannot be recognized by the anti-GLS antibodies to generate the close complex necessary for singlet oxygen to excite the acceptor bead, and no Alpha signal is emitted. (B) Optimization of Antibodies. HCT116 cells were trypsinized, resuspended in PBS, and heated for 3 minutes at 54°C prior to being lysed. Lysates were added to the noted anti-GLS antibodies in 384-well plates with a mixture of donor and acceptor beads. (C) Optimization of temperatures. HCT116 cells were treated with 10 μM CB-839 for 2 hours, then exposed to the noted temperatures for 3 minutes. (D): Characterization of concentration-dependent engagement of CB-839 to GLS. Unheated samples have baseline alpha signal, because GLS remains intact. This alpha signal does not significantly change with increasing concentrations of CB-839. However, heated samples have no alpha signal with very low CB-839 concentrations; the alpha signal increases with increased CB-839 concentrations, suggesting thermal protective effect with drug engagement of GLS. (E) Optimal duration of cell treatment. Cells were treated with 10 μM CB-839 for the noted durations; heating conditions: 52°C for 3 minutes. (F) CB-839 specifically engages GLS to provide thermal stability. HCT116 cells were treated with 10uM of compounds noted, all known to inhibit other enzymes; only CB-839 provided thermal protection of GLS.
Cetsa Cell Lysis Buffer 2, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cetsa cell lysis buffer 2/product/Revvity
Average 91 stars, based on 1 article reviews
cetsa cell lysis buffer 2 - by Bioz Stars, 2026-06
91/100 stars
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array buffer 1  (R&D Systems)
93
R&D Systems array buffer 1
(A) Schematic of AlphaLISA-based <t>CETSA.</t> GLS engagement by CB-839 protects it from thermal denaturation, which allows it to be recognized by GLS antibodies in 384-well plates. Secondary antibodies conjugated to donor and acceptor beads recognize the Fc domain of GLS antibodies, and all together form a complex. When donor beads are excited at 680nm, they emit singlet oxygen; the close complex formed by donor bead-antibody-GLS-antibody-acceptor bead allows the singlet oxygen to excite the acceptor bead, and an Alpha signal is emitted at 615nm. In contrast, if no CB-839 is present to engage with GLS, GLS is thermally denatured and precipitates, and cannot be recognized by the anti-GLS antibodies to generate the close complex necessary for singlet oxygen to excite the acceptor bead, and no Alpha signal is emitted. (B) Optimization of Antibodies. HCT116 cells were trypsinized, resuspended in PBS, and heated for 3 minutes at 54°C prior to being lysed. Lysates were added to the noted anti-GLS antibodies in 384-well plates with a mixture of donor and acceptor beads. (C) Optimization of temperatures. HCT116 cells were treated with 10 μM CB-839 for 2 hours, then exposed to the noted temperatures for 3 minutes. (D): Characterization of concentration-dependent engagement of CB-839 to GLS. Unheated samples have baseline alpha signal, because GLS remains intact. This alpha signal does not significantly change with increasing concentrations of CB-839. However, heated samples have no alpha signal with very low CB-839 concentrations; the alpha signal increases with increased CB-839 concentrations, suggesting thermal protective effect with drug engagement of GLS. (E) Optimal duration of cell treatment. Cells were treated with 10 μM CB-839 for the noted durations; heating conditions: 52°C for 3 minutes. (F) CB-839 specifically engages GLS to provide thermal stability. HCT116 cells were treated with 10uM of compounds noted, all known to inhibit other enzymes; only CB-839 provided thermal protection of GLS.
Array Buffer 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/array buffer 1/product/R&D Systems
Average 93 stars, based on 1 article reviews
array buffer 1 - by Bioz Stars, 2026-06
93/100 stars
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lepr biotin  (R&D Systems)
94
R&D Systems lepr biotin
(A) Schematic of AlphaLISA-based <t>CETSA.</t> GLS engagement by CB-839 protects it from thermal denaturation, which allows it to be recognized by GLS antibodies in 384-well plates. Secondary antibodies conjugated to donor and acceptor beads recognize the Fc domain of GLS antibodies, and all together form a complex. When donor beads are excited at 680nm, they emit singlet oxygen; the close complex formed by donor bead-antibody-GLS-antibody-acceptor bead allows the singlet oxygen to excite the acceptor bead, and an Alpha signal is emitted at 615nm. In contrast, if no CB-839 is present to engage with GLS, GLS is thermally denatured and precipitates, and cannot be recognized by the anti-GLS antibodies to generate the close complex necessary for singlet oxygen to excite the acceptor bead, and no Alpha signal is emitted. (B) Optimization of Antibodies. HCT116 cells were trypsinized, resuspended in PBS, and heated for 3 minutes at 54°C prior to being lysed. Lysates were added to the noted anti-GLS antibodies in 384-well plates with a mixture of donor and acceptor beads. (C) Optimization of temperatures. HCT116 cells were treated with 10 μM CB-839 for 2 hours, then exposed to the noted temperatures for 3 minutes. (D): Characterization of concentration-dependent engagement of CB-839 to GLS. Unheated samples have baseline alpha signal, because GLS remains intact. This alpha signal does not significantly change with increasing concentrations of CB-839. However, heated samples have no alpha signal with very low CB-839 concentrations; the alpha signal increases with increased CB-839 concentrations, suggesting thermal protective effect with drug engagement of GLS. (E) Optimal duration of cell treatment. Cells were treated with 10 μM CB-839 for the noted durations; heating conditions: 52°C for 3 minutes. (F) CB-839 specifically engages GLS to provide thermal stability. HCT116 cells were treated with 10uM of compounds noted, all known to inhibit other enzymes; only CB-839 provided thermal protection of GLS.
Lepr Biotin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lepr biotin/product/R&D Systems
Average 94 stars, based on 1 article reviews
lepr biotin - by Bioz Stars, 2026-06
94/100 stars
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wells  (R&D Systems)
94
R&D Systems wells
(A) Schematic of AlphaLISA-based <t>CETSA.</t> GLS engagement by CB-839 protects it from thermal denaturation, which allows it to be recognized by GLS antibodies in 384-well plates. Secondary antibodies conjugated to donor and acceptor beads recognize the Fc domain of GLS antibodies, and all together form a complex. When donor beads are excited at 680nm, they emit singlet oxygen; the close complex formed by donor bead-antibody-GLS-antibody-acceptor bead allows the singlet oxygen to excite the acceptor bead, and an Alpha signal is emitted at 615nm. In contrast, if no CB-839 is present to engage with GLS, GLS is thermally denatured and precipitates, and cannot be recognized by the anti-GLS antibodies to generate the close complex necessary for singlet oxygen to excite the acceptor bead, and no Alpha signal is emitted. (B) Optimization of Antibodies. HCT116 cells were trypsinized, resuspended in PBS, and heated for 3 minutes at 54°C prior to being lysed. Lysates were added to the noted anti-GLS antibodies in 384-well plates with a mixture of donor and acceptor beads. (C) Optimization of temperatures. HCT116 cells were treated with 10 μM CB-839 for 2 hours, then exposed to the noted temperatures for 3 minutes. (D): Characterization of concentration-dependent engagement of CB-839 to GLS. Unheated samples have baseline alpha signal, because GLS remains intact. This alpha signal does not significantly change with increasing concentrations of CB-839. However, heated samples have no alpha signal with very low CB-839 concentrations; the alpha signal increases with increased CB-839 concentrations, suggesting thermal protective effect with drug engagement of GLS. (E) Optimal duration of cell treatment. Cells were treated with 10 μM CB-839 for the noted durations; heating conditions: 52°C for 3 minutes. (F) CB-839 specifically engages GLS to provide thermal stability. HCT116 cells were treated with 10uM of compounds noted, all known to inhibit other enzymes; only CB-839 provided thermal protection of GLS.
Wells, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wells/product/R&D Systems
Average 94 stars, based on 1 article reviews
wells - by Bioz Stars, 2026-06
94/100 stars
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cell lysis buffer 1 cat no 890713  (R&D Systems)
91
R&D Systems cell lysis buffer 1 cat no 890713
(A) Schematic of AlphaLISA-based <t>CETSA.</t> GLS engagement by CB-839 protects it from thermal denaturation, which allows it to be recognized by GLS antibodies in 384-well plates. Secondary antibodies conjugated to donor and acceptor beads recognize the Fc domain of GLS antibodies, and all together form a complex. When donor beads are excited at 680nm, they emit singlet oxygen; the close complex formed by donor bead-antibody-GLS-antibody-acceptor bead allows the singlet oxygen to excite the acceptor bead, and an Alpha signal is emitted at 615nm. In contrast, if no CB-839 is present to engage with GLS, GLS is thermally denatured and precipitates, and cannot be recognized by the anti-GLS antibodies to generate the close complex necessary for singlet oxygen to excite the acceptor bead, and no Alpha signal is emitted. (B) Optimization of Antibodies. HCT116 cells were trypsinized, resuspended in PBS, and heated for 3 minutes at 54°C prior to being lysed. Lysates were added to the noted anti-GLS antibodies in 384-well plates with a mixture of donor and acceptor beads. (C) Optimization of temperatures. HCT116 cells were treated with 10 μM CB-839 for 2 hours, then exposed to the noted temperatures for 3 minutes. (D): Characterization of concentration-dependent engagement of CB-839 to GLS. Unheated samples have baseline alpha signal, because GLS remains intact. This alpha signal does not significantly change with increasing concentrations of CB-839. However, heated samples have no alpha signal with very low CB-839 concentrations; the alpha signal increases with increased CB-839 concentrations, suggesting thermal protective effect with drug engagement of GLS. (E) Optimal duration of cell treatment. Cells were treated with 10 μM CB-839 for the noted durations; heating conditions: 52°C for 3 minutes. (F) CB-839 specifically engages GLS to provide thermal stability. HCT116 cells were treated with 10uM of compounds noted, all known to inhibit other enzymes; only CB-839 provided thermal protection of GLS.
Cell Lysis Buffer 1 Cat No 890713, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cell lysis buffer 1 cat no 890713/product/R&D Systems
Average 91 stars, based on 1 article reviews
cell lysis buffer 1 cat no 890713 - by Bioz Stars, 2026-06
91/100 stars
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liter 1  (Millipore)
90
Millipore liter 1
(A) Schematic of AlphaLISA-based <t>CETSA.</t> GLS engagement by CB-839 protects it from thermal denaturation, which allows it to be recognized by GLS antibodies in 384-well plates. Secondary antibodies conjugated to donor and acceptor beads recognize the Fc domain of GLS antibodies, and all together form a complex. When donor beads are excited at 680nm, they emit singlet oxygen; the close complex formed by donor bead-antibody-GLS-antibody-acceptor bead allows the singlet oxygen to excite the acceptor bead, and an Alpha signal is emitted at 615nm. In contrast, if no CB-839 is present to engage with GLS, GLS is thermally denatured and precipitates, and cannot be recognized by the anti-GLS antibodies to generate the close complex necessary for singlet oxygen to excite the acceptor bead, and no Alpha signal is emitted. (B) Optimization of Antibodies. HCT116 cells were trypsinized, resuspended in PBS, and heated for 3 minutes at 54°C prior to being lysed. Lysates were added to the noted anti-GLS antibodies in 384-well plates with a mixture of donor and acceptor beads. (C) Optimization of temperatures. HCT116 cells were treated with 10 μM CB-839 for 2 hours, then exposed to the noted temperatures for 3 minutes. (D): Characterization of concentration-dependent engagement of CB-839 to GLS. Unheated samples have baseline alpha signal, because GLS remains intact. This alpha signal does not significantly change with increasing concentrations of CB-839. However, heated samples have no alpha signal with very low CB-839 concentrations; the alpha signal increases with increased CB-839 concentrations, suggesting thermal protective effect with drug engagement of GLS. (E) Optimal duration of cell treatment. Cells were treated with 10 μM CB-839 for the noted durations; heating conditions: 52°C for 3 minutes. (F) CB-839 specifically engages GLS to provide thermal stability. HCT116 cells were treated with 10uM of compounds noted, all known to inhibit other enzymes; only CB-839 provided thermal protection of GLS.
Liter 1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/liter 1/product/Millipore
Average 90 stars, based on 1 article reviews
liter 1 - by Bioz Stars, 2026-06
90/100 stars
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30x epigenetics buffer supplement  (Revvity)
90
Revvity 30x epigenetics buffer supplement
(A) Schematic of AlphaLISA-based <t>CETSA.</t> GLS engagement by CB-839 protects it from thermal denaturation, which allows it to be recognized by GLS antibodies in 384-well plates. Secondary antibodies conjugated to donor and acceptor beads recognize the Fc domain of GLS antibodies, and all together form a complex. When donor beads are excited at 680nm, they emit singlet oxygen; the close complex formed by donor bead-antibody-GLS-antibody-acceptor bead allows the singlet oxygen to excite the acceptor bead, and an Alpha signal is emitted at 615nm. In contrast, if no CB-839 is present to engage with GLS, GLS is thermally denatured and precipitates, and cannot be recognized by the anti-GLS antibodies to generate the close complex necessary for singlet oxygen to excite the acceptor bead, and no Alpha signal is emitted. (B) Optimization of Antibodies. HCT116 cells were trypsinized, resuspended in PBS, and heated for 3 minutes at 54°C prior to being lysed. Lysates were added to the noted anti-GLS antibodies in 384-well plates with a mixture of donor and acceptor beads. (C) Optimization of temperatures. HCT116 cells were treated with 10 μM CB-839 for 2 hours, then exposed to the noted temperatures for 3 minutes. (D): Characterization of concentration-dependent engagement of CB-839 to GLS. Unheated samples have baseline alpha signal, because GLS remains intact. This alpha signal does not significantly change with increasing concentrations of CB-839. However, heated samples have no alpha signal with very low CB-839 concentrations; the alpha signal increases with increased CB-839 concentrations, suggesting thermal protective effect with drug engagement of GLS. (E) Optimal duration of cell treatment. Cells were treated with 10 μM CB-839 for the noted durations; heating conditions: 52°C for 3 minutes. (F) CB-839 specifically engages GLS to provide thermal stability. HCT116 cells were treated with 10uM of compounds noted, all known to inhibit other enzymes; only CB-839 provided thermal protection of GLS.
30x Epigenetics Buffer Supplement, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/30x epigenetics buffer supplement/product/Revvity
Average 90 stars, based on 1 article reviews
30x epigenetics buffer supplement - by Bioz Stars, 2026-06
90/100 stars
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rbc lysis buffer 1  (Revvity)
90
Revvity rbc lysis buffer 1
(A) Schematic of AlphaLISA-based <t>CETSA.</t> GLS engagement by CB-839 protects it from thermal denaturation, which allows it to be recognized by GLS antibodies in 384-well plates. Secondary antibodies conjugated to donor and acceptor beads recognize the Fc domain of GLS antibodies, and all together form a complex. When donor beads are excited at 680nm, they emit singlet oxygen; the close complex formed by donor bead-antibody-GLS-antibody-acceptor bead allows the singlet oxygen to excite the acceptor bead, and an Alpha signal is emitted at 615nm. In contrast, if no CB-839 is present to engage with GLS, GLS is thermally denatured and precipitates, and cannot be recognized by the anti-GLS antibodies to generate the close complex necessary for singlet oxygen to excite the acceptor bead, and no Alpha signal is emitted. (B) Optimization of Antibodies. HCT116 cells were trypsinized, resuspended in PBS, and heated for 3 minutes at 54°C prior to being lysed. Lysates were added to the noted anti-GLS antibodies in 384-well plates with a mixture of donor and acceptor beads. (C) Optimization of temperatures. HCT116 cells were treated with 10 μM CB-839 for 2 hours, then exposed to the noted temperatures for 3 minutes. (D): Characterization of concentration-dependent engagement of CB-839 to GLS. Unheated samples have baseline alpha signal, because GLS remains intact. This alpha signal does not significantly change with increasing concentrations of CB-839. However, heated samples have no alpha signal with very low CB-839 concentrations; the alpha signal increases with increased CB-839 concentrations, suggesting thermal protective effect with drug engagement of GLS. (E) Optimal duration of cell treatment. Cells were treated with 10 μM CB-839 for the noted durations; heating conditions: 52°C for 3 minutes. (F) CB-839 specifically engages GLS to provide thermal stability. HCT116 cells were treated with 10uM of compounds noted, all known to inhibit other enzymes; only CB-839 provided thermal protection of GLS.
Rbc Lysis Buffer 1, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rbc lysis buffer 1/product/Revvity
Average 90 stars, based on 1 article reviews
rbc lysis buffer 1 - by Bioz Stars, 2026-06
90/100 stars
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Image Search Results


(A) Schematic of AlphaLISA-based CETSA. GLS engagement by CB-839 protects it from thermal denaturation, which allows it to be recognized by GLS antibodies in 384-well plates. Secondary antibodies conjugated to donor and acceptor beads recognize the Fc domain of GLS antibodies, and all together form a complex. When donor beads are excited at 680nm, they emit singlet oxygen; the close complex formed by donor bead-antibody-GLS-antibody-acceptor bead allows the singlet oxygen to excite the acceptor bead, and an Alpha signal is emitted at 615nm. In contrast, if no CB-839 is present to engage with GLS, GLS is thermally denatured and precipitates, and cannot be recognized by the anti-GLS antibodies to generate the close complex necessary for singlet oxygen to excite the acceptor bead, and no Alpha signal is emitted. (B) Optimization of Antibodies. HCT116 cells were trypsinized, resuspended in PBS, and heated for 3 minutes at 54°C prior to being lysed. Lysates were added to the noted anti-GLS antibodies in 384-well plates with a mixture of donor and acceptor beads. (C) Optimization of temperatures. HCT116 cells were treated with 10 μM CB-839 for 2 hours, then exposed to the noted temperatures for 3 minutes. (D): Characterization of concentration-dependent engagement of CB-839 to GLS. Unheated samples have baseline alpha signal, because GLS remains intact. This alpha signal does not significantly change with increasing concentrations of CB-839. However, heated samples have no alpha signal with very low CB-839 concentrations; the alpha signal increases with increased CB-839 concentrations, suggesting thermal protective effect with drug engagement of GLS. (E) Optimal duration of cell treatment. Cells were treated with 10 μM CB-839 for the noted durations; heating conditions: 52°C for 3 minutes. (F) CB-839 specifically engages GLS to provide thermal stability. HCT116 cells were treated with 10uM of compounds noted, all known to inhibit other enzymes; only CB-839 provided thermal protection of GLS.

Journal: Journal of genetics and genomics = Yi chuan xue bao

Article Title: A facile and sensitive method of quantifying glutaminase binding to its inhibitor CB-839 in tissues

doi: 10.1016/j.jgg.2020.06.001

Figure Lengend Snippet: (A) Schematic of AlphaLISA-based CETSA. GLS engagement by CB-839 protects it from thermal denaturation, which allows it to be recognized by GLS antibodies in 384-well plates. Secondary antibodies conjugated to donor and acceptor beads recognize the Fc domain of GLS antibodies, and all together form a complex. When donor beads are excited at 680nm, they emit singlet oxygen; the close complex formed by donor bead-antibody-GLS-antibody-acceptor bead allows the singlet oxygen to excite the acceptor bead, and an Alpha signal is emitted at 615nm. In contrast, if no CB-839 is present to engage with GLS, GLS is thermally denatured and precipitates, and cannot be recognized by the anti-GLS antibodies to generate the close complex necessary for singlet oxygen to excite the acceptor bead, and no Alpha signal is emitted. (B) Optimization of Antibodies. HCT116 cells were trypsinized, resuspended in PBS, and heated for 3 minutes at 54°C prior to being lysed. Lysates were added to the noted anti-GLS antibodies in 384-well plates with a mixture of donor and acceptor beads. (C) Optimization of temperatures. HCT116 cells were treated with 10 μM CB-839 for 2 hours, then exposed to the noted temperatures for 3 minutes. (D): Characterization of concentration-dependent engagement of CB-839 to GLS. Unheated samples have baseline alpha signal, because GLS remains intact. This alpha signal does not significantly change with increasing concentrations of CB-839. However, heated samples have no alpha signal with very low CB-839 concentrations; the alpha signal increases with increased CB-839 concentrations, suggesting thermal protective effect with drug engagement of GLS. (E) Optimal duration of cell treatment. Cells were treated with 10 μM CB-839 for the noted durations; heating conditions: 52°C for 3 minutes. (F) CB-839 specifically engages GLS to provide thermal stability. HCT116 cells were treated with 10uM of compounds noted, all known to inhibit other enzymes; only CB-839 provided thermal protection of GLS.

Article Snippet: For AlphaLisa-based CETSA, cells were lysed in CETSA Cell Lysis Buffer 2 (PerkinElmer, USA, CETSA-BUF2-100ML).

Techniques: Concentration Assay